Alloxan mgkg, ip was used for the induction of diabetes in adult male wistar rats. Oral administration of ocimum sanctum extracts for 14 days showed antidiabetic potential against alloxan diabetes induced alterations in the level of sgot, sgpt, alp and bilirubin. Mice used for toxicity and rats for antidiabetic study. Andiroba oil on wound healing in rats with induced diabetes. Diabetes induction in rats the alloxan is widely used to induce diabetes type i in the. Comparison of streptozotocin and alloxaninduced diabetes in the rat, including volumetric quantitation of the pancreatic islets, volume 9, issue3, pp 178184 cite as comparison of streptozotocin and alloxaninduced diabetes in the rat, including volumetric quantitation of the pancreatic islets diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Effects of andiroba oil carapa guianensis on wound. To elucidate the pharmacological basis of the antidiabetic efficacy of this plant drug, the effect of r. Effect of ocimum sanctum against alloxan induced diabetes. Alloxaninduced diabetes, a common model for evaluating the. Effects of ece on fasting blood glucose of normal and diabetic rats diabetes is characterized by hyperglycemia with an increase of glucose in blood. Effect of shilajit on blood glucose and lipid profile in. Antidiabetic effect of ipomoea batatas in normal and. Alloxan diabetes definition of alloxan diabetes by.
Beauv annonaceae offers alternative treatment for diabetes mellitus. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on. Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a. Silymarin is a freeradical scavenger and a membrane stabilizer which prevents lipoperoxidation and its associated cell damage in some experimental models. Fresh liver weight and its relationship with body weight were obtained, and liver tissue was. Antioxidant and prebiotic effects of a beverage composed.
Diabetes was induced in albino rats by single intraperitoneal injection of alloxan 70mgkg body weight. Anion gap toxicity in alloxan induced type 2 diabetic rats treated with. The root extract was the best one for reduction of serum glucose followed by leaves then fruits. Lakshmi bhavani department of botany, university college of science saifabad, osmania university, hyderabad 500004, telangana, india. Of immediate interest was the question of whether permanent diabetes could be produced in other animals. Group i constituted the control group and group ii constituted the alloxaninduced diabetic group. In vitro and in vivo antidiabetic activity of the leaves of ravenala madagascariensis sonn. Also, this convenient method of producing a permanent experimental pancreatic diabetes has stimulated research in the fields of carbohydrate metabolism and the role of insulin in metabolism. The following results were reported from other studies. Diabetes was induced by interperitoneal injection of alloxan monohydrate 150 mgkg bw followed by a booster dose of 70 mgkg bw 48 hours after first induction.
Effect of shilajit on blood glucose and lipid profile in alloxan induced diabetic rats n. After 72 h, those with plasma glucose levels 200 mgdl were classified as diabetic. Alloxan has been administered in single or multiple. Group 1 rats served as the normal control, group 2 served as the caraway control, whereas group 3 rats. Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxaninduced diabetic rats. Alloxaninduced diabetes mellitus how is alloxaninduced.
Antidiabetic activity on extracts of mangifera indica in alloxan monohydrate induced diabetic rats july, 2011 drug invention today, 2011, 37, 165168 167 equipotent in blood sugar reductions. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Hypoglycemic and antioxidant effects of leaf essential oil of. Journal of diabetes and endocrinology antihyperglycaemic. Antihyperglycemic effect of gasca d herbal formulation on. Alloxan definition of alloxan by medical dictionary. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Thirty 30 adults male wistar rats were randomly divided into six 6 groups of five 5 each based on their body weight.
Hypoglycaemic effect of galactooligosaccharides in alloxaninduced. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Experimental models play an important role in understanding such a disease, which is treatable only. The mechanisms of alloxan and streptozotocininduced.
We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Cardiac performance was assessed at 7, 30, 100, 180, 240, and 360 days after induction of diabetes using the isolated perfused working heart technique. The synergistic action of dehydroascorbic acid and alloxan in producing a diabetes mellituslike state in the laboratory rat is utilized. Effect of alloxaninduced diabetes on implantation sites. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. Hypoglycemic effects in alloxaninduced diabetic rats of.
Study on antihyperglycemic effect of lycopene in alloxan. Managing diabetes devoid of any adverse effect remains an issue to health system. This study describes a rat diabetes mellitus model induced by administering a reduced dose of alloxan, thus greatly reducing the animals death rate. Pdf antidiabetic and hypolipidemic effect of asparagus. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Alloxaninduced diabetes causes morphological and ultrastructural. How to induce diabetes in rats with alloxan diabetestalk. Antidiabetic effects of the ethanolic root extract of. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxan induced diabetic rats. Pdf alloxaninduced diabetes, a common model for evaluating the.
Alloxaninduced diabetes, a common model for evaluating. Diabetes was induced by alloxan monohydrate 150mgkg i. Dec 18, 2007 alloxan has two distinct pathological effects. Metformin was metabolized via hepatic cyp2c11, 2d1, and 3a12 in rats. The mechanisms of alloxan and streptozotocininduced diabetes. Antidiabetic effect of ipomoea batatas in normal and alloxan. Notably, it is a referred, highly indexed, online international journal with high impact factor. Experimental model of induction of diabetes mellitus in rats. The animals were distributed into 4 groups of 5 rats each namely. We performed experiments with a rat model of stzinduced diabetes, which is commonly used, since it is similar to the. Insulin and alpha amylase levels in alloxaninduced diabetic. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Leaf extracts in alloxan induced diabetic rats syed mansoor ahmed, vrushabendra swamy bm, p gopkumar r dhanapal and vm chandrashekara department of pharmacology, m.
Jun 26, 2012 hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. The effect of alloxan and streptozotocininduced diabetes. Research article alloxan induced diabetes mellitus in wistar. Mar 18, 20 antidiabetic effects of turmeric in alloxan induce d diabetic rats article pdf available in journal of evolution of medical and dental sciences 211. A single intraperitoneal injection of 200 mgkg of alloxan into white rats causes typical diabetes mellitus. Effects of diabetes mellitus induced by alloxan on the. In research it is used for induction of type 1 diabetes.
Antihyperglycemic potential of teak seeds in alloxan induced diabetic rats html full text. Group a served as the control and received 1 mlkg body weight of 5% ethanol being solvent used, group b received 1 ml alloxan. In the present study, thirty six male sprague dawley rats. Hypoglycemic and antihyperlipidemic effects of adiantum caudatum in alloxan induced diabetes in rats article pdf available in asian journal of pharmaceutical and clinical research 91. Diabetes was induced in sprague dawley rats after overnight fast with 150 mgkg alloxan intraperitoneally.
Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Diabetes is a metabolic disease linked to weakened sugar metabolism. Sir, we read with interest the article titled anomalies in alloxaninduced diabetic model. Prevention of alloxaninduced diabetes mellitus in the rat by silymarin.
Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Pdf hypoglycemic and antihyperlipidemic effects of. Diabetes mellitus has been a menace to mankind from time immemorial. Pdf experimental diabetes induced by alloxan and streptozotocin. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. We report herein the findings of this research work. Diabetes was induced in rats by intravenous injection of alloxan monohydrate dissolved in normal saline into the dorsal tail vein at.
Normal diet supplemented with 10% and 20% of kernel cake of balanites aegyptiaca were fed to diabetic rats for three weeks. Results of this method, in which the diabetogenic agents are administered subcutaneously, are compared with those in a series of rats made diabetic by intravenously administered alloxan. Lower doses can result in autoreversion to normal state without medical help, while higher doses cause toxicity and loss of experimental animals. Alloxaninduced diabetes triggers the development of. Pdf protective role of phyllanthus fraternus in alloxan. The histologic changes are necrosis and complete disappearance of the beta cells from the pancreatic islets.
Diabetes was induced with administration of alloxan, 150 mgkg body weight i. Alloxan induced changes in the collagen characteristics in the skin of male garden lizards, calotes versicolor, from three age groups archives of gerontology and geriatrics, vol. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Alloxan diabetes definition of alloxan diabetes by medical. Female wistar rats were made diabetic by a single i. Diabetes was induced by administration of alloxan 100 mgkg and rats were. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. It has been proposed that lipid peroxidation caused by free radicals may be involved in. Coadministration of alloxan and nicotinamide in rats produces. Open access treatment of alloxaninduced diabetic rats with. Indeed, the essential oil of rosescented geranium leaves was prepared and their antioxidant activities as well as their hypoglycemic effects were studied. Diabetes was induced in wistar rats n 25 by intravenous administration of alloxan 42 mgkg and were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction. The mechanism of alloxan and streptozotocin action in b cells.
This research was designed to investigate antihyperglycaemic impact of aqueous extract of moringa oleifera leaf on alloxaninduced diabetic male wistar rats. Comparative effects of rothmannia hispida leaves extract and. Carotid arteries were extracted from rats, carefully dissected from surrounding tissue, cut into 4 mmlong rings and placed in an organ bath. Antidiabetic activity on extracts of mangifera indica in. Both normal positive control group 2 and diabetic treated group 4 rats received gasca d herbal formulation at a. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxaninduced diabetic rats.
Alloxaninduced diabetes alters rat common carotid artery. In alloxan induced albino rats rajaram k department of biotechnology, bharathidasan institute of technology, anna university, tiruchirappalli620 024, tamil nadu, india. Frontiers antihyperglycemic and antihyperlipidemic. Some antidiabetic properties of prosopis farcta extracts in. Isolated working hearts from diabetic rats have a decreased ability to respond to increasing preload or afterload. Alloxaninduced diabetes in rats and the effects of black. Diabetes was induced in the experimental rats with alloxan 70 mg kg1 body weight. This research was designed to investigate antihyperglycaemic impact of aqueous extract of moringa oleifera leaf on alloxan induced diabetic male wistar rats. Pdf prevention of alloxaninduced diabetes mellitus in. This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. Hearts were obtained from female wistar rats 120 days or 7 days after the induction of diabetes by a single i. Jun 22, 2005 this study was undertaken to investigation the effect of diasulin, a poly herbal drug composed of ethanolic extract of ten medicinal plants on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in alloxan induced diabetes.
With experience it is possible to produce diabetes in greater than. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis. The effects of balanite aegyptiaca kernel cake as supplement. The mechanisms by which alloxan monohydrate brings about its diabetic state include selective destruction of.
In the in vivo experiment, glucose given to rats 20 min prior to alloxan partially restricted alloxaninduced increase in the activity of glutathione peroxidase ec 1. Production of diabetes mellitus in rats with alloxan. Jayshree department of pharmacognosy, college of pharmacy, madras medical college, chennai600 003, india. Research article alloxan induced diabetes mellitus in. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. The mechanism of alloxan and streptozotocin action in b. It has been successfully induced in a variety of animal species. Studies on the administration of vernonia amygdalina del. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.
The current study involved two groups of male wistar rats 220280 g. Groups 2 4 in additional to control diet received alloxan treatment 150mgkg i. Thirty nondiabetic control rats nc and 30 untreated. The effect of chronic alloxan and streptozotocininduced. Experimental diabetes induced by alloxan and streptozotocin. Evaluation of body weight, serum glucose level and oxidative stress parameters of diabetic rats administered phenolic aqueous leaf extract of. Alloxan is selectively toxic to insulinproducing pancreatic beta cells because it preferentially accumulates in beta cells through uptake via the glut2 glucose transporter. Alloxaninduced diabetes delays repair in a rat model of closed tibial. The aqueous roots extract of murva was found to be safe at a dose of 2000mgkg. The effects of alloxan on the histology of the pancreas.
Pdf alloxaninduced diabetes triggers the development of. Analysis of certain biochemical indices on alloxan induced. Therefore, there is an increase in the search for better antidiabetic medicine. Hyperglycaemia was induced in each fasted rat by administering alloxan monohydrate 150 mg kg body weight. Article information, pdf download for coadministration of alloxan and nicotinamide in. Passiflora edulis, antidiabetic, hyperglycemia, alloxan mono hydrate introduction nsulin is the principal hormone secreted by. Hyperglycaemic rats were treated with or without oral hypoglycaemic drugs metformin, 150 mgkgd. Antidiabetic effects of the ethanolic root extract of uvaria. Evaluation of body weight, serum glucose level and oxidative stress. Hypoglycemic and antioxidant effects of leaf essential oil. Ethanolic extract of diasulin a, poly herbal drug was administered orally 200 mgkg body weight for 30 days. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxaninduced diabetic rats. Time course effect of alloxan on blood glucose level of rat.
Alloxan is a classical diabetogenic chemical which exerts selective cytotoxic in. In vitro and in vivo antidiabetic activity of the leaves. Frontiers antihyperglycemic and antihyperlipidemic effects. Antidiabetic and hypolipidemic effect of asparagus racemosus in alloxan induced diabetes in rats. In diabetes mellitus the body cannot secrete enough insulin or it cannot use its. Antihyperlipidemic and antiperoxidative effect of diasulin. Antihyperlipidemic and antiperoxidative effect of diasulin, a. Production of alloxan diabetes and ketoacidosis in the. The study aimed at evaluating antidiabetic activity of the ethanolic root extract of u. To study the effect of shilajit a herbomineral preparation on blood glucose and lipid profile in euglycemic and alloxan induced diabetic rats and its effects on the above parameters in. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is. Burk, rf 1976 glutathione peroxidase activity in selenium deficient rat liver. Alloxan was administered as a single dose 120 mgkg bw to induce diabetes.
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